Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
Identifieur interne : 002D71 ( Main/Exploration ); précédent : 002D70; suivant : 002D72Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
Auteurs : Jin-Yan Chen [République populaire de Chine] ; Wan-Nan Chen [République populaire de Chine] ; Kwok-Man Vincent Poon [République populaire de Chine] ; Bo-Jian Zheng [République populaire de Chine] ; Xu Lin [République populaire de Chine] ; Yong-Xiang Wang [République populaire de Chine] ; Yu-Mei Wen [République populaire de Chine]Source :
- Archives of Virology [ 0304-8608 ] ; 2009-03-01.
Descripteurs français
- KwdFr :
- Animaux, Cartographie d'interactions entre protéines, DEAD-box RNA helicases (métabolisme), Humains, Liaison aux protéines, Lignée cellulaire, Macaca mulatta, Methyltransferases (métabolisme), Rats, Réplication virale, Techniques de double hybride, Techniques de knock-down de gènes, Virus du SRAS (physiologie).
- MESH :
- Pascal (Inist)
English descriptors
- KwdEn :
- Animals, Cell Line, DEAD-box RNA Helicases (metabolism), Gene Knockdown Techniques, Humans, Macaca mulatta, Mammalia, Methyltransferases (metabolism), Protein, Protein Binding, Protein Interaction Mapping, Rats, SARS Virus (physiology), Severe acute respiratory syndrome virus, Two-Hybrid System Techniques, Virus Replication, Yeast.
- MESH :
- chemical , metabolism : DEAD-box RNA Helicases, Methyltransferases.
- physiology : SARS Virus.
- Animals, Cell Line, Gene Knockdown Techniques, Humans, Macaca mulatta, Protein Binding, Protein Interaction Mapping, Rats, Two-Hybrid System Techniques, Virus Replication.
Abstract
Abstract: To reveal the putative cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the yeast two-hybrid system. Positively interacting proteins were further tested using a mammalian cell hybrid system and co-immunoprecipitation in the human A549 cell line, which has been shown to support SARS coronavirus replication. Out of the seven positive clones observed by yeast two-hybrid assay, only the Ddx5 (Asp-Glu-Ala-Asp box polypeptide 5) protein showed specific interaction with SARS-CoV helicase. When expression of DdX5 was knocked down by small interfering RNA (siRNA), SARS coronavirus replication was significantly inhibited in fetal rhesus kidney (FRhK-4) cells. Since Ddx5 is a multifunctional protein that plays important roles in transcriptional regulation, its interaction with SARS coronavirus helicase provides interesting clues for studying virus–host cell interactions in SARS-CoV infections.
Url:
- https://api.istex.fr/ark:/67375/VQC-LXRDC3L5-S/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087151
DOI: 10.1007/s00705-009-0323-y
Affiliations:
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Le document en format XML
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ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: To reveal the putative cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the yeast two-hybrid system. Positively interacting proteins were further tested using a mammalian cell hybrid system and co-immunoprecipitation in the human A549 cell line, which has been shown to support SARS coronavirus replication. Out of the seven positive clones observed by yeast two-hybrid assay, only the Ddx5 (Asp-Glu-Ala-Asp box polypeptide 5) protein showed specific interaction with SARS-CoV helicase. When expression of DdX5 was knocked down by small interfering RNA (siRNA), SARS coronavirus replication was significantly inhibited in fetal rhesus kidney (FRhK-4) cells. Since Ddx5 is a multifunctional protein that plays important roles in transcriptional regulation, its interaction with SARS coronavirus helicase provides interesting clues for studying virus–host cell interactions in SARS-CoV infections.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Fujian</li></region><settlement><li>Fuzhou</li></settlement></list><tree><country name="République populaire de Chine"><region name="Fujian"><name sortKey="Chen, Jin Yan" sort="Chen, Jin Yan" uniqKey="Chen J" first="Jin-Yan" last="Chen">Jin-Yan Chen</name></region><name sortKey="Chen, Wan Nan" sort="Chen, Wan Nan" uniqKey="Chen W" first="Wan-Nan" last="Chen">Wan-Nan Chen</name><name sortKey="Lin, Xu" sort="Lin, Xu" uniqKey="Lin X" first="Xu" last="Lin">Xu Lin</name><name sortKey="Poon, Kwok Man Vincent" sort="Poon, Kwok Man Vincent" uniqKey="Poon K" first="Kwok-Man Vincent" last="Poon">Kwok-Man Vincent Poon</name><name sortKey="Wang, Yong Xiang" sort="Wang, Yong Xiang" uniqKey="Wang Y" first="Yong-Xiang" last="Wang">Yong-Xiang Wang</name><name sortKey="Wen, Yu Mei" sort="Wen, Yu Mei" uniqKey="Wen Y" first="Yu-Mei" last="Wen">Yu-Mei Wen</name><name sortKey="Wen, Yu Mei" sort="Wen, Yu Mei" uniqKey="Wen Y" first="Yu-Mei" last="Wen">Yu-Mei Wen</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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